Laparoscopic bowel injury in an animal model: monocyte migration and apoptosis.
Surg Endosc
; 19(4): 484-7, 2005 Apr.
Article
em En
| MEDLINE
| ID: mdl-15696363
ABSTRACT
BACKGROUND:
Unrecognized laparoscopic bowel injury has a delayed and covert presentation. Differences in monocyte migration and apoptosis between laparoscopic and open bowel injury were determined.METHODS:
For this study, 24 rabbits were divided into laparoscopic (n = 9) and open surgical (n = 9) bowel injury groups and a control group (n = 6) without bowel injury. Bowel injury was created using monopolar electrocautery. The animals were killed 1 day, 1 week, and 2 weeks after surgery. Monocyte migration assay was performed across a modified Boyden chamber. Apoptosis was assessed by DNA fluorescent stain H-33342.RESULTS:
In laparoscopy, monocyte apoptosis was decreased (p < 0.001), and migration was increased (p < 0.05), as compared with the open group. Apoptosis increased over time in both study groups, and was higher than in the control group (p < 0.001). Migration was decreased in both study groups as compared with the control group (p < 0.05)CONCLUSIONS:
These results suggest decreased immune system priming with laparoscopic bowel injury, which may contribute to the masking of relevant signs and symptoms of peritonitis.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Queimaduras por Corrente Elétrica
/
Monócitos
/
Laparoscopia
/
Eletrocoagulação
/
Intestinos
/
Complicações Intraoperatórias
/
Macrófagos
Tipo de estudo:
Etiology_studies
/
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Surg Endosc
Assunto da revista:
DIAGNOSTICO POR IMAGEM
/
GASTROENTEROLOGIA
Ano de publicação:
2005
Tipo de documento:
Article
País de afiliação:
Estados Unidos