eNOS genotype is without effect on circulating nitrite/nitrate level in healthy male population.
Thromb Res
; 115(5): 375-9, 2005.
Article
em En
| MEDLINE
| ID: mdl-15733970
ABSTRACT
INTRODUCTION:
Nitric oxide (NO) plays an important role in the regulation of the cardiovascular system. It is produced by endothelial nitric oxide synthase (eNOS), which exhibits genetic polymorphisms. Although the clinically relevant polymorphism T(+IhI-786)C reduces eNOS-promoter activity, it is not clear whether circulating nitrite/nitrate (NOx) are affected by this polymorphism. MATERIALS ANDMETHODS:
We addressed this issue by studying a homogeneous group of 200 healthy subjects (males, Caucasians, nonsmokers, 18+IBM-56 years of age, and not taking any medication). Genotypes were determined by restriction fragment length polymorphism and circulating NOx were determined by chemiluminescence.RESULTS:
We found nonsignificant effects of the T(+IhI-786)C polymorphism on circulating NOx (mean+ALE-S.D.=52.2+ALE-21.4, 49.0+ALE-17.8, and 45.9+ALE-16.8 +A7w-mol/L for genotypes +IBw-TT,+IB0 +IBw-TC,+IB0 and +IBw-CC,+IB0 respectively) and on total plasma cholesterol concentrations (both P>.05). No correlation was found between circulating NOx and total plasma cholesterol concentrations (P>.05).CONCLUSIONS:
Our study provides strong evidence that the T(+IhI-786)C polymorphism does not affect plasma NOx concentrations, which are believed to reflect endogenous production of NO. Therefore, our results suggest that this polymorphism does not affect endogenous NO production.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
População
/
Óxido Nítrico Sintase
/
População Branca
/
Nitratos
/
Nitritos
Limite:
Adolescent
/
Adult
/
Humans
/
Male
/
Middle aged
País/Região como assunto:
America do sul
/
Brasil
Idioma:
En
Revista:
Thromb Res
Ano de publicação:
2005
Tipo de documento:
Article
País de afiliação:
Brasil