The L-type calcium channel alpha 1C subunit gene undergoes extensive, uncoordinated alternative splicing.
Mol Cell Biochem
; 269(1-2): 153-63, 2005 Jan.
Article
em En
| MEDLINE
| ID: mdl-15786728
ABSTRACT
The alpha1C subunit is the pore-forming protein for the L-type calcium channel. Previous studies indicate that there is possible tissue-specific alternative splicing of this gene. In this study we cloned the entire open reading frame of the alpha1C subunit cDNA from adult rat cardiac myocytes in a single piece (6.64 kb). Using 75 positive clones that were identified by restriction enzyme mapping, we tested the alternative splicing patterns of the Ca(v) 1.2 gene that encodes the alpha1C subunit protein and focused on five loci IS6, post-IS6, IIIS2, IVS3, and the c-terminus. The results indicate that (1) alternative splicing occurs in most of the loci, giving rise to two or three different isoforms at those sites; (2) there is a predominant form for each splicing site, (3) there does not appear to be consistent coordination of splicing at multiple loci of this gene. Alternative splicing is not tissue-specific in most regions.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Processamento Alternativo
/
Canais de Cálcio Tipo L
/
Sítios de Splice de RNA
Limite:
Animals
Idioma:
En
Revista:
Mol Cell Biochem
Ano de publicação:
2005
Tipo de documento:
Article
País de afiliação:
Estados Unidos