[Isolation and identification of a gene: attractin related to inhibiting erythroid differentiation in K562 cells].

ABSTRACT

Differential display PCR method was used to isolate four differential display fragments between the wild type K562 cell line and the variant type K562 cell line that expressed the human beta-globin gene. After sequencing, the most remarkable different fragment,named dd1,was selected for further study. The analysis of RT-PCR and Northern blot hybridization showed that dd1 was exactly the differentiation fragment between the two cell lines. The homology analysis indicated that dd1 was matched to Attractin (GeneBank registration No.AF106861). It might be an adhesion receptor related to inhibiting erythroid differentiation based on its structure.