Analysis of 686 expressed sequence tags (ESTs) from the intertidal harpacticoid copepod Tigriopus japonicus (Crustacea, Copepoda).
Mar Pollut Bull
; 51(8-12): 757-68, 2005.
Article
em En
| MEDLINE
| ID: mdl-16291190
ABSTRACT
The intertidal harpacticoid copepod Tigriopus japonicus is an important species in the study of marine pollution. To facilitate molecular biomonitoring using T. japonicus, we constructed a T. japonicus unidirectional cDNA library using lambdaZAP expression vector, excised to pBluescript vector with the aid of helper phage, and analyzed 686 randomly picked expressed sequence tags (ESTs) from this species. From the 686 ESTs sequenced, we found several functional genes such as vitellin, kinases and potential detoxification-related genes. We are now preparing a T. japonicus cDNA chip for molecular ecotoxicological studies. In this paper, we discuss the potential use of T. japonicus ESTs and their importance in ecotoxicology.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Expressão Gênica
/
Monitoramento Ambiental
/
Etiquetas de Sequências Expressas
/
Copépodes
Limite:
Animals
Idioma:
En
Revista:
Mar Pollut Bull
Ano de publicação:
2005
Tipo de documento:
Article
País de afiliação:
Coréia do Sul