Microsatellite markers associated with resistance to Marek's disease in commercial layer chickens.
Poult Sci
; 84(11): 1678-88, 2005 Nov.
Article
em En
| MEDLINE
| ID: mdl-16463964
ABSTRACT
The objective of the current study was to identify QTL conferring resistance to Marek's disease (MD) in commercial layer chickens. To generate the resource population, 2 partially inbred lines that differed in MD-caused mortality were intermated to produce 5 backcross families. Vaccinated chicks were challenged with very virulent plus (vv+) MD virus strain 648A at 6 d and monitored for MD symptoms. A recent field isolate of the MD virus was used because the lines were resistant to commonly used older laboratory strains. Selective genotyping was employed using 81 microsatellites selected based on prior results with selective DNA pooling. Linear regression and Cox proportional hazard models were used to detect associations between marker genotypes and survival. Significance thresholds were validated by simulation. Seven and 6 markers were significant based on proportion of false positive and false discovery rate thresholds less than 0.2, respectively. Seventeen markers were associated with MD survival considering a comparison-wise error rate of 0.10, which is about twice the number expected by chance, indicating that at least some of the associations represent true effects. Thus, the present study shows that loci affecting MD resistance can be mapped in commercial layer lines. More comprehensive studies are under way to confirm and extend these results.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Galinhas
/
Doença de Marek
/
Repetições de Microssatélites
Tipo de estudo:
Prognostic_studies
/
Risk_factors_studies
Limite:
Animals
Idioma:
En
Revista:
Poult Sci
Ano de publicação:
2005
Tipo de documento:
Article
País de afiliação:
Estados Unidos