Expression of discoidin domain receptor 1 during mouse brain development follows the progress of myelination.
Neuroscience
; 140(2): 463-75, 2006 Jun 30.
Article
em En
| MEDLINE
| ID: mdl-16603319
ABSTRACT
Discoidin domain receptor 1 is a tyrosine kinase receptor expressed in a variety of tissues including the brain. This study describes mRNA and protein expression of discoidin domain receptor 1 in mouse brain during development and provides new insights into its role during gliogenesis and neurogenesis. We performed in situ hybridization for discoidin domain receptor 1 in mouse brains at embryonic day 18, postnatal days 5, 9, 15, 21 and adulthood and observed a diffuse pattern in the proliferative areas during embryogenesis. From postnatal day 5 onwards, a defined cellular expression pattern of discoidin domain receptor 1 was observed, mainly located in white matter tracts and following a spatio-temporal pattern that overlapped the progress of myelination. Next, we performed double-labeling reactions (in situ hybridization followed by immunohistochemistry) that confirmed that discoidin domain receptor 1 was expressed by mature oligodendrocytes. We observed that cells positive for discoidin domain receptor 1 also expressed carnosine and anti-adenomatous polyposis coli, two mature oligodendrocyte markers. Based on the localization of discoidin domain receptor 1 specifically in the white matter fiber tracts during postnatal development, we suggest that discoidin domain receptor 1 participates in the development and maintenance of the myelin sheath.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Encéfalo
/
Receptores Mitogênicos
/
Diferenciação Celular
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Receptores Proteína Tirosina Quinases
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Bainha de Mielina
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Fibras Nervosas Mielinizadas
Limite:
Animals
Idioma:
En
Revista:
Neuroscience
Ano de publicação:
2006
Tipo de documento:
Article
País de afiliação:
Espanha