Binding of a histidine-rich peptide to Porphyromonas gingivalis.
FEMS Microbiol Lett
; 66(3): 253-6, 1991 Aug 15.
Article
em En
| MEDLINE
| ID: mdl-1663059
ABSTRACT
Porphyromonas gingivalis 381 cells were incubated with 125I-histidine-rich polypeptide (histatin) 5 in the presence or absence of unlabeled histatin 5, to evaluate the histatin-binding capacity of the cells. The binding of histatin 5 was rapid, reversible, saturable and specific. The number of histatin 5-binding sites per cell was 3,600, and the dissociation constant (Kd) was in the order of 10(-6) M. These findings suggest that histatin interacts with certain bacterial cells through specific binding sites on their surface, and will allow the development of a histatin radioreceptor assay.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas e Peptídeos Salivares
/
Porphyromonas gingivalis
Idioma:
En
Revista:
FEMS Microbiol Lett
Ano de publicação:
1991
Tipo de documento:
Article
País de afiliação:
Japão