[The effect of oncolytic adenovirus on human umbilical vein endothelial cell].
Sheng Wu Gong Cheng Xue Bao
; 22(3): 397-402, 2006 May.
Article
em Zh
| MEDLINE
| ID: mdl-16755917
ABSTRACT
The E1A gene was obtained by PCR with QBI-293A cell genome DNA as template. After enzyme digestion, the E1A gene was ligated to transfer vector pAdTrack-CMV. The positive clone pAdTrack-CMV-E1A were lineared by PmeI and co-transformed with pAdEasy-1 in BJ5183 E. coli. The recombinant adenovirus vector pAdEasy-1-pAdTrack-CMV-E1A were digested by PacI and transfected into QBI-293A cells with liposomes. The oncolytic recombinant adenovirus Ad-E1A was obtained after 7 days. The results showed that this oncolytic adenovirus Ad-E1A can replicate in ECV304 cells and inhibit growth of ECV304 cell. In addition, it also decreased the secretion of VEGF and expression of NF-kappaB of ECV304 cells, indicating that Ad-E1A have potential of inhibition of tumor metastasis.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Veias Umbilicais
/
Adenoviridae
/
Proteínas E1A de Adenovirus
/
Células Endoteliais
/
Vírus Oncolíticos
Limite:
Humans
Idioma:
Zh
Revista:
Sheng Wu Gong Cheng Xue Bao
Assunto da revista:
BIOTECNOLOGIA
Ano de publicação:
2006
Tipo de documento:
Article
País de afiliação:
China