Oxidized low-density lipoprotein antagonizes the activation of purified soluble guanylate cyclase by endothelium-derived relaxing factor but does not interfere with its biosynthesis.
Cell Signal
; 3(4): 361-7, 1991.
Article
em En
| MEDLINE
| ID: mdl-1681884
ABSTRACT
Oxidized low-density lipoprotein (LDLox) is a molecule with strong atherogenic properties. In a concentration dependent fashion, LDLox antagonized the activation of purified soluble guanylate cyclase by endothelium-derived relaxing factor (EDRF), which was produced in vitro by incubation of a partially purified EDRF-forming enzyme in the presence of L-arginine, Ca2+ and NADPH. The inhibitory effect of LDLox was potentiated by preincubation of the soluble guanylate cyclase with LDLox, but not when the EDRF-forming enzyme was pretreated with LDLox. As LDLox did not diminish the calmodulin-dependent conversion of L-arginine into L-citrulline by the EDRF-forming enzyme it would appear that EDRF-biosynthesis was not affected by LDLox. It is suggested that the impaired relaxant response of atherosclerotic blood vessels to endothelium-dependent vasodilators was not due to a reduced formation of EDRF but due to a diminished responsiveness of soluble guanylate cyclase.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Guanilato Ciclase
/
Lipoproteínas LDL
/
Óxido Nítrico
Limite:
Animals
/
Female
/
Humans
/
Male
Idioma:
En
Revista:
Cell Signal
Ano de publicação:
1991
Tipo de documento:
Article
País de afiliação:
Áustria