Identification and characterization of functional CD154 (CD40 ligand) in the Pekin duck.
Dev Comp Immunol
; 31(1): 61-71, 2007.
Article
em En
| MEDLINE
| ID: mdl-16837046
ABSTRACT
Binding of CD154, a member of the TNF ligand superfamily, to its receptor CD40 is essential for the development and regulation of adaptive immune responses in mammals. The duck CD154 (DuCD154) encoding gene was isolated from activated splenocytes using RT-PCR. Sequence analysis of the cloned DuCD154 gene revealed an open reading frame of 819 base pairs encoding a 272 amino acid protein. The extracellular domain of DuCD154 was identified and expressed for characterization and generation of antibodies. DuCD154 mRNA was predominantly expressed in spleen, thymus and duodenum. DuCD154 protein generated in cell culture was secreted and formed dimers. DuCD154 markedly enhanced proliferative responses in duck splenocytes when used alone or in conjunction with LPS or PHA. These observations suggest that DuCD154 has functional equivalence with mammalian CD154 and that the central role of CD154 as an immunoregulatory protein had already evolved before the divergence of birds and mammals.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ligante de CD40
/
Patos
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Animals
Idioma:
En
Revista:
Dev Comp Immunol
Ano de publicação:
2007
Tipo de documento:
Article