Isolation and characterization of human embryonic stem cells.
Methods Mol Biol
; 331: 55-76, 2006.
Article
em En
| MEDLINE
| ID: mdl-16881509
ABSTRACT
The derivation of human embryonic stem (hES) cells is a challenging procedure. The isolation and maintenance of hES is visually and manually complicated, involving mechanical or enzymatic passaging using either collagenase or trypsin. This chapter describes detailed protocols that have been used for the derivation, maintenance, and characterization of hES cells in vitro along with protocols to test their differentiation potential in vivo. When used as a guideline, these protocols will assist researchers in setting up a hES cell laboratory.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Separação Celular
/
Técnicas de Cultura de Células
/
Células-Tronco Pluripotentes
Limite:
Humans
Idioma:
En
Revista:
Methods Mol Biol
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
2006
Tipo de documento:
Article
País de afiliação:
Estados Unidos