Crystallization and preliminary diffraction studies of recombinant human granulocyte-stimulating factor (KW2228).
J Mol Biol
; 214(1): 25-6, 1990 Jul 05.
Article
em En
| MEDLINE
| ID: mdl-1695250
ABSTRACT
Human granulocyte colony-stimulating factor (hG-CSF) specifically stimulates proliferation of neutrophils. Two crystal forms of a mutant of hG-CSF expressed in Escherichia coli have been obtained using the hanging drop vapour diffusion method. One form is triclinic, space group P1, with cell dimensions a = 37.3 A, b = 46.4 A, c = 47.7 A, alpha = 105.5 degrees, beta = 98.0 degrees and gamma = 109.4 degrees. The other is monoclinic, space group C2, with cell dimensions a = 82.0 A, b = 49.2 A, c = 49.4 A and beta = 113.9 degrees. Both crystal forms diffract beyond 2.0 A and are suitable for X-ray analysis.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fatores Estimuladores de Colônias
Limite:
Humans
Idioma:
En
Revista:
J Mol Biol
Ano de publicação:
1990
Tipo de documento:
Article
País de afiliação:
Japão