A high-throughput monolithic HPLC method for rapid vitamin C phenotyping of berry fruit.
Phytochem Anal
; 17(5): 284-90, 2006.
Article
em En
| MEDLINE
| ID: mdl-17019929
ABSTRACT
A rapid method for the quantification of L-ascorbic acid (1) in berry fruit by HPLC with photodiode array detection is presented. L-Ascorbic acid was resolved on a C18 monolithic column with aqueous buffer, after which the column was washed with acetonitrile to remove lipophilic compounds prior to re-equilibration for analysis of the next sample. Using the monolithic column format with high mobile phase flow rates, the entire separation, wash and re-equilibration were achieved in 3 min. With the exception of gooseberry (Ribes uva-crispa), for which an interfering compound co-eluted, concentrations of 1 could be determined in a wide range of berry fruits after extraction in metaphosphoric acid without further sample preparation. Using this extraction method, recoveries of 1 in excess of 85% were achieved. Fruit or juice extracts were stable in 5% metaphosphoric acid for at least 4 h and stability could be extended to longer than 150 h by the addition of the reducing agent tris(2-carboxethyl)phosphine hydrochloride. Following validation, the method was utilised for the phenotyping of fruit in a Scottish Crop Research Institute (SCRI) Ribes nigrum L. breeding population of 300 individuals. An improved extraction method allowed extraction, quantification of 1 and data analysis to be undertaken in less than one working week.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ácido Ascórbico
/
Cromatografia Líquida de Alta Pressão
/
Ribes
/
Frutas
Idioma:
En
Revista:
Phytochem Anal
Assunto da revista:
BOTANICA
/
QUIMICA
Ano de publicação:
2006
Tipo de documento:
Article
País de afiliação:
Reino Unido