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Utility of gas chromatography for rapid identification of mycobacterial species frequently encountered in clinical laboratory.
Mosca, Adriana; Russo, Francesco; Miragliotta, Luisa; Iodice, Maria Antonietta; Miragliotta, Giuseppe.
Afiliação
  • Mosca A; Section of Microbiology, Department of Clinical Medicine, Immunology and Infectious Diseases, University of Bari, Bari, Italy.
J Microbiol Methods ; 68(2): 392-5, 2007 Feb.
Article em En | MEDLINE | ID: mdl-17098312
ABSTRACT
Over the last years, the clinical importance of mycobacteria has been raised. In this regard, it is important their identification in order to establish either the clinical significance or the appropriate therapy of the disease. Biochemical tests are usually time consuming until the report of results, that is why more rapid techniques are needed. As an alternative identification method, we have used a commercially available system for microbial identification based on whole cellular fatty acids analysis using gas-chromatography (GC). Sixty-eight strains of Mycobacterium tuberculosis, Mycobacterium gordonae, Mycobacterium xenopi, Mycobacterium kansasii, Mycobacterium fortuitum, and Mycobacterium avium-intracellulare were clearly identified by their unique fatty acid profile using the Sherlock Microbial Identification System (MIS). The results were in agreement with those obtained with traditional methods. This method is highly automated, rapid, easy to perform with a sample preparation for lipid analysis which is neither time consuming nor requiring a particular expertise. On this basis the MIS-GC method for the identification of some clinically important mycobacteria appears to be suitable for routine clinical use.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Cromatografia Gasosa / Ácidos Graxos / Mycobacterium / Infecções por Mycobacterium Tipo de estudo: Diagnostic_studies Limite: Humans Idioma: En Revista: J Microbiol Methods Ano de publicação: 2007 Tipo de documento: Article País de afiliação: Itália
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Cromatografia Gasosa / Ácidos Graxos / Mycobacterium / Infecções por Mycobacterium Tipo de estudo: Diagnostic_studies Limite: Humans Idioma: En Revista: J Microbiol Methods Ano de publicação: 2007 Tipo de documento: Article País de afiliação: Itália