The importance of careful blood processing in isolation of cell-free DNA.
Ann N Y Acad Sci
; 1075: 313-7, 2006 Sep.
Article
em En
| MEDLINE
| ID: mdl-17108226
ABSTRACT
In healthy individuals, the source of cell-free plasma DNA is predominantly apoptotic, whereas, increased plasma DNA integrity is seen in cancer patients. Therefore, it is important to carefully isolate absolutely "cell-free" plasma DNA. Plasma DNA from 30 healthy females was analyzed using 4 PCR amplicons of increasing size, comparing standard blood processing with additional centrifugation steps prior to DNA extraction. Cellular DNA contamination, indicated by positive amplicons >300 bp was eliminated only after the extra centrifugation step. This highlights the importance of careful processing in preparation of cell-free plasma DNA as a tool for cancer detection and we recommend the use of a microcentrifuge spin, prior to DNA extraction.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Análise Química do Sangue
/
DNA
Limite:
Female
/
Humans
Idioma:
En
Revista:
Ann N Y Acad Sci
Ano de publicação:
2006
Tipo de documento:
Article
País de afiliação:
Reino Unido