Ultrastructural morphometry of mammary gland in transgenic and non-transgenic rabbits.
Anat Histol Embryol
; 35(6): 351-6, 2006 Dec.
Article
em En
| MEDLINE
| ID: mdl-17156086
ABSTRACT
The mammary gland of transgenic animals has been used for the production of recombinant proteins of therapeutic and nutraceutical use. The objective of this study was to compare the ultrastructure of transgenic and non-transgenic rabbit mammary gland tissue. New Zealand White transgenic rabbits were obtained by breeding non-transgenic rabbits with transgenic founder rabbits containing a whey acidic protein-human factor VIII (WAP-hFVIII) transgene integrated into their genome. Samples of mammary gland tissue from lactating rabbit females were isolated by surgical procedures. These samples were examined by optical and electron microscopy and photographs were taken. Measurements of ultrastructural organelles were made from digital images of the mammary cells. No differences were found in the cellular structure of mammary tissue, but significant differences t((0.001)) in the relative volume of mitochondria and vacuoles between transgenic and non-transgenic mammary gland epithelium were observed.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Coelhos
/
Animais Geneticamente Modificados
/
Glândulas Mamárias Animais
Limite:
Animals
Idioma:
En
Revista:
Anat Histol Embryol
Ano de publicação:
2006
Tipo de documento:
Article