Derivation of mixed phenotype cell lines with ras-myc- and raf-myc-containing retroviral vectors after infection of murine long-term bone marrow cultures.
Exp Hematol
; 19(7): 664-8, 1991 Aug.
Article
em En
| MEDLINE
| ID: mdl-1716589
ABSTRACT
Murine long-term bone marrow cultures were infected with retroviral vectors expressing the viral ras, raf, and myc oncogenes, alone and in combination. Stably transformed clonal cell lines were obtained after infection with ras-myc and raf-myc retroviruses but not by vectors expressing ras, myc, or raf alone. Northern blot analysis demonstrated that the two clonal cell lines expressed high levels of vector-specific transcripts. Phenotypic analysis of the cell lines by flow microfluorimetry and histochemical staining suggested that both cell lines expressed markers associated with cells of the megakaryocyte differentiation pathway. Histochemical staining demonstrated that these cell lines also expressed cytoplasmic enzymes associated with granulocytes and/or monocytes/macrophages. These cell lines, despite their clonal origin, are therefore of a mixed phenotype.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fenótipo
/
Retroviridae
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Células da Medula Óssea
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Transfecção
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Genes myc
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Genes ras
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Proteínas Proto-Oncogênicas
Limite:
Animals
Idioma:
En
Revista:
Exp Hematol
Ano de publicação:
1991
Tipo de documento:
Article