Transgenic expression and recovery of biologically active recombinant human insulin from Arabidopsis thaliana seeds.
Plant Biotechnol J
; 4(1): 77-85, 2006 Jan.
Article
em En
| MEDLINE
| ID: mdl-17177787
ABSTRACT
The increased incidence of diabetes, coupled with the introduction of alternative delivery methods that rely on higher doses, is expected to result in a substantial escalation in the demand for affordable insulin in the future. Limitations in the capacity and economics of production will make it difficult for current manufacturing technologies to meet this demand. We have developed a novel expression and recovery technology for the economical manufacture of biopharmaceuticals from oilseeds. Using this technology, recombinant human precursor insulin was expressed in transgenic plants. Plant-derived insulin accumulates to significant levels in transgenic seed (0.13% total seed protein) and can be enzymatically treated in vitro to generate a product with a mass identical to that of the predicted product, DesB(30)-insulin. The biological activity of this product in vivo and in vitro was demonstrated using an insulin tolerance test in mice and phosphorylation assay performed in a mammalian cell culture system, respectively.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Sementes
/
Engenharia Genética
/
Arabidopsis
/
Insulina
Limite:
Animals
/
Humans
/
Male
Idioma:
En
Revista:
Plant Biotechnol J
Assunto da revista:
BIOTECNOLOGIA
/
BOTANICA
Ano de publicação:
2006
Tipo de documento:
Article
País de afiliação:
Canadá