Application of capillary zone electrophoresis to the characterisation of the human milk protein profile and its evolution throughout lactation.
J Chromatogr A
; 1146(1): 110-7, 2007 Mar 30.
Article
em En
| MEDLINE
| ID: mdl-17320885
ABSTRACT
This work describes the use of capillary zone electrophoresis for the characterisation of human milk proteins. The major proteins were identified following different strategies, such as the treatment with enzymes for selective protein modification. Using this method we studied the proteins in human milk from different donors throughout lactation. Qualitative and quantitative differences in the composition of the individual proteins were observed. The different beta-casein phosphoforms were separated and quantified. The average proportion of the 0P1P2P3P4P5P was, approximately, 3694102. The evolution of the ratio of the different beta-casein phosphoforms during lactation is reported.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Lactação
/
Eletroforese Capilar
/
Leite Humano
/
Proteínas do Leite
Tipo de estudo:
Prognostic_studies
/
Qualitative_research
Limite:
Female
/
Humans
Idioma:
En
Revista:
J Chromatogr A
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
Espanha