Flow cytometric detection of saxitoxins using fluorescent voltage-sensitive dyes.
Anal Biochem
; 366(2): 149-55, 2007 Jul 15.
Article
em En
| MEDLINE
| ID: mdl-17512490
ABSTRACT
By virtue of their ability to block depolarization of nerve cells, the saxitoxins exert the toxic effects associated with paralytic shellfish poisoning and allow for their detection through various methodologies. When veratridine-induced depolarization is followed using voltage-sensitive fluorescent dyes, the presence of these toxic blocking agents can be observed as a decrease in fluorescence of dye-treated nerve cells. Detection using flow cytometry provides for selection of the most responsive population of cultured mouse neuroblastoma (Neuro 2a) cells thereby enhancing assay sensitivity and this approach can be accomplished in real time. The method is demonstrated in preliminary studies using saxitoxin and crude shellfish extracts.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Saxitoxina
/
Citometria de Fluxo
/
Corantes Fluorescentes
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Revista:
Anal Biochem
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
Estados Unidos