Prion infection correlates with hypersensitivity of P2X7 nucleotide receptor in a mouse microglial cell line.
FEBS Lett
; 581(16): 3019-26, 2007 Jun 26.
Article
em En
| MEDLINE
| ID: mdl-17544414
We recently established mouse microglial cells persistently infected with mouse-adapted scrapie ME7 (ScMG20/ME7) for in vitro study of prion pathogenesis. Here, we found that ScMG20/ME7 cells were hypersensitive to P2X7 receptor agonists, as demonstrated by sustained Ca(2+) influx, membrane pore formation, cell death, and interleukin-1beta release. P2X7 mRNA expression was upregulated in these cells, and also in scrapie-infected mice brains. Treatment with pentosan polysulfate eliminated the infectivity and disease-related forms of prion protein from ScMG20/ME7 cell cultures, however, hypersensitivity of P2X7 receptors remained. These results suggest that prion infections may strongly affect the P2X7 receptor system in mouse microglial cells.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Scrapie
/
Príons
/
Trifosfato de Adenosina
/
Microglia
/
Agonistas do Receptor Purinérgico P2
Limite:
Animals
Idioma:
En
Revista:
FEBS Lett
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
Japão