Characterization and isolation of some genes of the shikimate pathway in sensitive and resistant Centaurea jacea plants after ozone exposure.
Environ Pollut
; 151(2): 272-9, 2008 Jan.
Article
em En
| MEDLINE
| ID: mdl-17706847
ABSTRACT
Centaurea jacea has been suggested as a potential bioindicator for ozone, but little is known about its intraspecific variation in sensitivity, especially at molecular level. The effects of ozone (200 ppb, 5 h) on sensitive and resistant lines of Centaurea have been investigated at the end of fumigation. Sensitive plants showed characteristic symptoms of injury in the form of diffuse discoloration stipples on leaves. A PCR-based approach was used to identify and isolate a partial-length cDNA coding for PAL and CHS genes. The northern analysis of PAL showed accumulation of transcript in both lines correlated with a typical increase of PAL activity (+41 and +91% in resistant and sensitive material, respectively, compared to controls). On the contrary, the transcripts of CHS, in resistant and sensitive plants, did not change after treatment. Total phenols were not affected by ozone, while anthocyanins were quickly utilised by resistant clone as antioxidant compounds.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ozônio
/
Ácido Chiquímico
/
Transdução de Sinais
/
Regulação da Expressão Gênica de Plantas
/
Centaurea
/
Poluentes Atmosféricos
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Idioma:
En
Revista:
Environ Pollut
Assunto da revista:
SAUDE AMBIENTAL
Ano de publicação:
2008
Tipo de documento:
Article
País de afiliação:
Itália