[Effect of reperfusion leukocyte-depleted blood on canine myocardial energy metabolism balance during cardiopulmonary bypass].
Sichuan Da Xue Xue Bao Yi Xue Ban
; 38(4): 671-4, 2007 Jul.
Article
em Zh
| MEDLINE
| ID: mdl-17718438
ABSTRACT
OBJECTIVE:
To investigate the impact of myocardial energy metabolism of canine reperfused with leukocyte-depleted blood during cardiopulmonary bypass (CPB).METHODS:
Eighteen adult healthy dogs undergoing CPB were randomly divided into 3 groups the control group (group C, n=6), whole blood (group W, n=6) and the experimental group (group L, n=6) with use of the leukocyte depletion filter (LDF) on the bypass circuit. The contents of adenine nucleotide, superoxide dismutase (SOD), glutathione peroxidase (GSH-PX), myeloperoxidase (MPO), malondialdehyde (MDA) and mitochondrial swelling of myocardia were determined respectively before cross-clamping, at 40 min and 60 min after aortic cross-clamping (AC), 30 min and 60 min after declamping (DC) during CPB.RESULTS:
Reperfused with leukocyte-depleted blood by LDF connected with bypass circuit, the dog hearts of group L at 60 min after AC, 30 min and 60 min after DC were much better in the recovery of myocardium energy metabolism, higher in contents of myocardium SOD and GSH-PX than those in group C and W (P < 0.01). The myocardium MPO, MDA and mitochondrial swelling degree at 60 min after AC, 30 min and 60 min after DC were distinctly lower in group L than those in group C and W (P < 0.01).CONCLUSION:
Myocardium has serious energy exhaustion and deteriorated metabolism during CPB. Myocardial mitochondrial structure and function can be protected and myocardial energy depletion can be reduced by infusion of leukocyte-depleted blood to the heart before DC, which can distinctly attenuate myocardial ischemia/reperfusion injury.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ponte Cardiopulmonar
/
Reperfusão
/
Metabolismo Energético
/
Leucócitos
/
Miocárdio
Limite:
Animals
Idioma:
Zh
Revista:
Sichuan Da Xue Xue Bao Yi Xue Ban
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
China