High-performance liquid chromatography post-column derivatization with fluorescence detection to study the influence of ambroxol on dipalmitoylphosphatidylcholine levels in rabbit eustachian tube washings.
J Chromatogr
; 553(1-2): 1-6, 1991 Aug 16.
Article
em En
| MEDLINE
| ID: mdl-1787145
ABSTRACT
In this work an appropriate high-performance liquid chromatography method was set up to guarantee specificity, sensitivity, precision and accuracy in analyzing dipalmitoylphosphatidylcholine (DPPC) in rabbit eustachian tube washings, as well as to determine its varying levels after administration of ambroxol chloride. The procedure is based on a post-column derivatization with fluorescence detection using 1,6-diphenyl-1,3,5-hexatriene which exhibits increased fluorescence in a lipid environment. DPPC was chromatographed on a Hypersil C18. The mobile phase for the isocratic elution consisted of 40 mmol/l choline chloride in methanol-tetrahydrofuran (973). Ambroxol was given to a group of New Zealand white rabbits at a dose of 30 mg/kg. A second group receiving vehicle only acted as controls.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
1,2-Dipalmitoilfosfatidilcolina
/
Cromatografia Líquida de Alta Pressão
/
Tuba Auditiva
/
Ambroxol
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Revista:
J Chromatogr
Ano de publicação:
1991
Tipo de documento:
Article
País de afiliação:
Itália