[Experimental study on effect of Tongxinluo on nerve cell apoptosis after cerebral ischemia in middle cerebral arterial obstructive model rats].
Zhongguo Zhong Xi Yi Jie He Za Zhi
; 27(8): 720-3, 2007 Aug.
Article
em Zh
| MEDLINE
| ID: mdl-17879537
ABSTRACT
OBJECTIVE:
To explore the action mechanism of Tongxinluo Capsule (TXL) in protecting brain from ischemic damage.METHODS:
SD rats were divided into five groups randomly, the sham operation group, the model group, the MK-801 group, the large and low dosage TXL groups (TXLL and TXLS). After the middle cerebral arterial obstructive (MCAO) model was established, peritoneal injection of MK-801 0.5 mg/kg per day was given to the MK-801 group, and 1.0 g/(kg x d) and 0.5 g/(kg x d) of TXL powder was administered in twice via gastrogavage to the two TXL groups respectively. The nerve cell apoptosis rate, protein and mRNA expressions of Caspase-3, p53 and heat shock protein (HSP70) were observed using flow cytometry, Western blot and RT-PCR technique.RESULTS:
Both TXL and MK-801 could obviously lower the apoptosis rate in model rat (P < 0.05, P < 0.01), TXLL showed the optimal effect. Caspase-3, p53 protein and mRNA expression in the model group were obviously higher than those in the sham operated group. As compared with the model group, the expressions of Caspase-3 and p53 were lower and those of HSP70 and mRNA were higher in the two TXL and MK-801 groups (P < 0.05 or P < 0.01).CONCLUSION:
TXL displays it brain protective effect through reducing nerve cell apoptosis rate in MCAO model rats, the mechanism may be related to its actions in inhibiting apoptosis related factors Caspase-3 and p53, and promoting stress protecting factor HSP70.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Arteriopatias Oclusivas
/
Medicamentos de Ervas Chinesas
/
Apoptose
/
Fármacos Neuroprotetores
/
Neurônios
Tipo de estudo:
Etiology_studies
/
Prognostic_studies
Limite:
Animals
Idioma:
Zh
Revista:
Zhongguo Zhong Xi Yi Jie He Za Zhi
Assunto da revista:
TERAPIAS COMPLEMENTARES
Ano de publicação:
2007
Tipo de documento:
Article