Characterization of primary renal carcinoma cultures.
Urol Int
; 79(3): 235-43, 2007.
Article
em En
| MEDLINE
| ID: mdl-17940356
ABSTRACT
PURPOSE:
For a better understanding of the factors contributing to tumor progression in metastatic renal cell carcinoma and to identify possible targets for immunotherapeutic approaches, we characterized several primary cultures from renal cell carcinoma. MATERIALS ANDMETHODS:
Cell cultures were tested for activity of telomerase, secretion of immunosuppressive cytokines and others. The induction of cytotoxic activity against the autologous tumor was tested in a cytotoxicity assay after coculture of immunological effector cells with antigen-pulsed dendritic cells. The data were tested for influence on survival.RESULTS:
We were able to establish primary cell cultures from 58 patients with renal cell carcinoma and their metastasis. 48/58 were positive for telomerase activity and all secreted IL-6, TGF-beta, VEGF and IL-8. High TGF-beta secretion, the activity of telomerase and the induction of a telomerase-specific immune response against telomerase peptides in telomerase-positive tumors had a significant impact on survival.CONCLUSION:
TGF-beta secretion, activity of telomerase in telomerase-positive tumors and the ability to generate a telomerase-specific immune response might serve as a prognostic marker for RCC. New approaches might focus on attacking the TGF-beta pathway and on induction of telomerase-specific immune cells.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Carcinoma de Células Renais
/
Biomarcadores Tumorais
/
Citocinas
/
Telomerase
/
Neoplasias Renais
Tipo de estudo:
Prognostic_studies
Limite:
Aged80
Idioma:
En
Revista:
Urol Int
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
Alemanha