Saturation of the secretory pathway by overexpression of a hookworm (Necator americanus) Protein (Na-ASP1).
Methods Mol Biol
; 389: 65-76, 2007.
Article
em En
| MEDLINE
| ID: mdl-17951635
ABSTRACT
Human hookworm infection is one of the most significant parasitic infections, and a leading global cause of anemia and malnutrition of adults and children in rural areas of the tropics and subtropics. Necator americanus secretory protein (Na-ASP1), which is a potential vaccine candidate against hookworm infections, has been expressed in Pichia pastoris. Na-ASP1 protein was expressed extracellulary by employing the leader sequence of the alpha-mating factor of Saccharomyces cerevisiae. Most of the protein produced by single copy clones was secreted outside the cell. The Na-ASP1 steady state mRNA levels of the clones were correlated to their Na-ASP1 gene copy number. However, increasing gene copy number of Na-ASP1 protein in P. pastoris saturated secretory capacity and therefore, decreased the amount of secreted protein in clones harboring multiple copies of Na-ASP1 gene.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ancylostomatoidea
/
Expressão Gênica
/
Proteínas de Helminto
Limite:
Animals
Idioma:
En
Revista:
Methods Mol Biol
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
2007
Tipo de documento:
Article