STED microscopy with continuous wave beams.
Nat Methods
; 4(11): 915-8, 2007 Nov.
Article
em En
| MEDLINE
| ID: mdl-17952088
ABSTRACT
We report stimulated emission depletion (STED) fluorescence microscopy with continuous wave (CW) laser beams. Lateral fluorescence confinement from the scanning focal spot delivered a resolution of 29-60 nm in the focal plane, corresponding to a 5-8-fold improvement over the diffraction barrier. Axial spot confinement increased the axial resolution by 3.5-fold. We observed three-dimensional (3D) subdiffraction resolution in 3D image stacks. Viable for fluorophores with low triplet yield, the use of CW light sources greatly simplifies the implementation of this concept of far-field fluorescence nanoscopy.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Óptica e Fotônica
/
Lasers
/
Microscopia de Fluorescência
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Nat Methods
Assunto da revista:
TECNICAS E PROCEDIMENTOS DE LABORATORIO
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
Alemanha