Kremen is required for neural crest induction in Xenopus and promotes LRP6-mediated Wnt signaling.
Development
; 134(23): 4255-63, 2007 Dec.
Article
em En
| MEDLINE
| ID: mdl-17978005
ABSTRACT
Kremen 1 and 2 (Krm1/2) are transmembrane receptors for Wnt antagonists of the Dickkopf (Dkk) family and function by inhibiting the Wnt co-receptors LRP5/6. Here we show that Krm2 functions independently from Dkks during neural crest (NC) induction in Xenopus. Krm2 is co-expressed with, and regulated by, canonical Wnts. Krm2 is differentially expressed in the NC, and morpholino-mediated Krm2 knockdown inhibits NC induction, which is mimicked by LRP6 depletion. Conversely, krm2 overexpression induces ectopic NC. Kremens bind to LRP6, promote its cell-surface localization and stimulate LRP6 signaling. Furthermore, Krm2 knockdown specifically reduces LRP6 protein levels in NC explants. The results indicate that in the absence of Dkks, Kremens activate Wnt/beta-catenin signaling through LRP6.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Xenopus
/
Receptores de LDL
/
Proteínas de Xenopus
/
Embrião não Mamífero
/
Proteínas Wnt
/
Proteínas de Membrana
/
Crista Neural
Limite:
Animals
/
Female
/
Humans
Idioma:
En
Revista:
Development
Assunto da revista:
BIOLOGIA
/
EMBRIOLOGIA
Ano de publicação:
2007
Tipo de documento:
Article
País de afiliação:
Alemanha