Expansion of circulating Foxp3+)D25bright CD4+ T cells during specific venom immunotherapy.
Clin Exp Allergy
; 38(2): 291-7, 2008 Feb.
Article
em En
| MEDLINE
| ID: mdl-18070166
ABSTRACT
BACKGROUND:
Venom immunotherapy (VIT) induces long-lasting immune tolerance to hymenoptera venom antigens, but the underlying mechanisms are not yet clarified. Regulatory T cells are thought to play an important role in allergic diseases and tolerance induction during specific immunotherapy.AIM:
Characterize longitudinally the impact of VIT on the pool of circulating regulatory T cells.METHODS:
Fourteen hymenoptera venom-allergic patients with severe reactions (grades III-IV) were studied before, 6 and 12 months after starting ultra-rush VIT. Freshly isolated peripheral blood mononuclear cells were surface stained with a panel of markers of T cell differentiation and intracellularly for CTLA-4 and Foxp3 and analysed by flow cytometry. foxp3 mRNA was quantified by real-time PCR. VIT responses were assessed by measuring specific IgG4 and IgE levels. Eleven individuals with no history of insect venom allergy were studied as controls.RESULTS:
VIT induces a significant progressive increase in both the proportion and the absolute numbers of regulatory T cells defined as CD25bright and/or Foxp3+ CD4+ T cells. These changes are not related to alterations in the expression of activation markers or imbalances in the naïve/memory T cell compartments. foxp3 mRNA levels also increased significantly during VIT. Of note, the increase in circulating regulatory T cell counts significantly correlates with the venom-specific IgG4/IgE ratio shift.CONCLUSION:
VIT is associated with a progressive expansion of circulating regulatory T cells, supporting a role for these cells in tolerance induction.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Venenos de Vespas
/
Venenos de Abelha
/
Dessensibilização Imunológica
/
Linfócitos T Reguladores
/
Himenópteros
/
Hipersensibilidade Imediata
/
Mordeduras e Picadas de Insetos
Limite:
Adolescent
/
Adult
/
Aged
/
Animals
/
Female
/
Humans
/
Male
/
Middle aged
Idioma:
En
Revista:
Clin Exp Allergy
Assunto da revista:
ALERGIA E IMUNOLOGIA
Ano de publicação:
2008
Tipo de documento:
Article
País de afiliação:
Portugal