[An effective method for site-directed mutagenesis in plasmids and cloning single-stranded DNA fragments]. / Effektivnyi metod napravlennogo vvedeniia mutatsii v plazmidy i klonirovaniia odnotiazhevykh fragmentov DNA.
Bioorg Khim
; 17(11): 1487-93, 1991 Nov.
Article
em Ru
| MEDLINE
| ID: mdl-1811543
ABSTRACT
A three primer variant of the earlier devised oligonucleotide-directed mutagenesis in plasmids is described, useful also for the fast cloning of single-stranded DNA products of the asymmetric polymerase chain reaction (PCR). Using this method for plasmid pHD-001-14-11, a 59 b. p. deletion and a 7 b. p. insertion were simultaneously introduced at 81% frequency, and the PCR-copied phage fd transcription terminator (26 b. p.) was inserted with the yield of 67%.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Plasmídeos
/
DNA de Cadeia Simples
/
Mutagênese Sítio-Dirigida
Idioma:
Ru
Revista:
Bioorg Khim
Ano de publicação:
1991
Tipo de documento:
Article