Pressure refilled polyacrylamide columns for the separation of oligonucleotides by capillary electrophoresis.
Electrophoresis
; 12(12): 1056-8, 1991 Dec.
Article
em En
| MEDLINE
| ID: mdl-1815958
ABSTRACT
The separation of oligonucleotides by capillary zone electrophoresis (CZE) was studied in fused silica separation capillaries filled by linear (noncrosslinked) polyacrylamide (PAA) solutions, introduced into the capillary from the stock by pressure after each analysis. The time-consuming in-capillary polymerization step could thus be avoided, and fast and reproducible repetition of the analyses was assured. The PAA concentrations varied within the range of 3-10% and both the reproducibility of the analyses and the stability of the solution in the capillary, with and without a chemically treated inner wall, were tested. Ferguson plots were used to assess the size selectivity of the separation.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oligonucleotídeos
/
Eletroforese em Gel de Poliacrilamida
Idioma:
En
Revista:
Electrophoresis
Ano de publicação:
1991
Tipo de documento:
Article