Haplotyping by double PCR amplification of specific alleles.
Biotechniques
; 10(4): 436, 438, 440, 1991 Apr.
Article
em En
| MEDLINE
| ID: mdl-1831030
ABSTRACT
Haplotypes are useful in population genetics and medicine. Determining haplotypes in the absence of DNA samples from appropriate family members can be difficult and laborious. We have developed a rapid and reproducible method for haplotyping an individual in the absence of relatives. The method utilizes pairs of allele-specific PCR primers to differentially amplify each haplotype. Four amplifications can distinguish the haplotypes produced by two biallelic polymorphisms.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Haplótipos
/
Reação em Cadeia da Polimerase
Limite:
Humans
Idioma:
En
Revista:
Biotechniques
Ano de publicação:
1991
Tipo de documento:
Article