Impact of column temperature and mobile phase components on selectivity of hydrophilic interaction chromatography (HILIC).
J Sep Sci
; 31(9): 1449-64, 2008 May.
Article
em En
| MEDLINE
| ID: mdl-18435508
ABSTRACT
The retention mechanism and chromatographic behavior for different polar analytes under hydrophilic interaction chromatography (HILIC) conditions have been studied by application of different mobile phases and stationary phases to various analytes at different temperatures. In addition to the commonly accepted mechanism of analyte liquid-liquid partitioning between mobile phase and water-enriched solvent layer which is partially immobilized onto the surface of the stationary phase, hydrogen-bonding, hydrophobic interaction, and ion-exchange interactions may also be involved. The predominant retention mechanism in HILIC separation is not always easily predictable. It can depend not only on the characteristics of the analytes but also on the selection of mobile and stationary phase compositions. The objective of this review is to evaluate the potential application of column temperature and mobile phase composition toward improving HILIC selectivity. The functional groups from analyte structures, stationary phase materials and organic mobile phase solvents will be highlighted.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Cromatografia Líquida
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
J Sep Sci
Ano de publicação:
2008
Tipo de documento:
Article
País de afiliação:
Estados Unidos