Synthesis of methyl 5-O-trans-feruloyl-alpha-L-arabinofuranoside and its use as a substrate to assess feruloyl esterase activity.
Anal Biochem
; 194(1): 25-33, 1991 Apr.
Article
em En
| MEDLINE
| ID: mdl-1867381
ABSTRACT
A synthetic scheme was developed for the production of methyl 5-O-trans-feruloyl-alpha-L-arabinofuranoside (FA-Ara) in gram quantities. This molecule accurately models the chemical attachment of ferulic acid to polysaccharides found in cell walls of plants in the Gramineae family. It is therefore a realistic substrate that can be used to monitor feruloyl esterase activity. Ultraviolet spectral analysis indicated that FA-Ara has an absorption maximum distinct from the hydrolytic product, ferulic acid (FA), over a wide range of solution pH values. The log molar extinction coefficient ranges from 4.16 to 4.36 for FA-Ara and 4.16 to 4.33 for FA depending upon the pH of the buffered solution. Consequently a convenient spectrophotometric assay can be utilized to monitor esterase activity. Three different methods were developed for using this model substrate to assess esterase activity, including thin-layer chromatography, a spectrophotometric assay, and the use of high-performance liquid chromatography.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Arabinose
/
Hidrolases de Éster Carboxílico
/
Ácidos Cumáricos
Idioma:
En
Revista:
Anal Biochem
Ano de publicação:
1991
Tipo de documento:
Article