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Detection and reporting beta-lactam resistance phenotypes in Escherichia coli and Klebsiella pneumoniae: a multicenter proficiency study in Spain.
Conejo, M Carmen; Mata, C; Navarro, F; Pascual, A.
Afiliação
  • Conejo MC; Departamento de Microbiología, Facultad de Medicina, Av. Sánchez Pizjuan S/N, 41009 Sevilla, Spain. cconejo@us.es
Diagn Microbiol Infect Dis ; 62(3): 317-25, 2008 Nov.
Article em En | MEDLINE | ID: mdl-18692340
ABSTRACT
The ability of 57 Spanish microbiology laboratories in detecting and reporting beta-lactam resistance phenotypes in Escherichia coli and Klebsiella pneumoniae was evaluated. Laboratories received 6 well-characterized isolates expressing the most widespread extended-spectrum beta-lactamases (ESBLs) in Spain (4 CTX-M type, 1 TEM type, and 1 SHV type), 3 isolates producing AmpC-type enzymes (2 plasmid mediated and 1 E. coli hyperproducing its chromosomal AmpC), and 3 quality control strains. Ninety-one percent of laboratories recognized all ESBL producers correctly, and therefore, low error rates were observed when testing cephalosporins and aztreonam. The highest error rates were observed with combinations of penicillin plus beta-lactamase inhibitor, although more than 60% of cases were due to the interpretation made by the microbiologists. Correct recognition of all AmpC beta-lactamase-producing strains occurred in only 47.4% of laboratories. These isolates were wrongly reported as ESBL producers and penicillinase hyperproducers in 7.6 % and 5.8% of cases, respectively. Detection of the AmpC-type phenotype by Spanish laboratories needs to be improved.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Beta-Lactamases / Testes de Sensibilidade Microbiana / Resistência beta-Lactâmica / Escherichia coli / Laboratórios Tipo de estudo: Clinical_trials / Diagnostic_studies Limite: Humans País/Região como assunto: Europa Idioma: En Revista: Diagn Microbiol Infect Dis Ano de publicação: 2008 Tipo de documento: Article País de afiliação: Espanha

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Beta-Lactamases / Testes de Sensibilidade Microbiana / Resistência beta-Lactâmica / Escherichia coli / Laboratórios Tipo de estudo: Clinical_trials / Diagnostic_studies Limite: Humans País/Região como assunto: Europa Idioma: En Revista: Diagn Microbiol Infect Dis Ano de publicação: 2008 Tipo de documento: Article País de afiliação: Espanha