Influenza B virus NS1-truncated mutants: live-attenuated vaccine approach.
J Virol
; 82(21): 10580-90, 2008 Nov.
Article
em En
| MEDLINE
| ID: mdl-18768976
ABSTRACT
Type B influenza viruses can cause substantial morbidity and mortality in the population, and vaccination remains by far the best means of protection against infections with these viruses. Here, we report the construction of mutant influenza B viruses for potential use as improved live-virus vaccine candidates. Employing reverse genetics, we altered the NS1 gene, which encodes a type I interferon (IFN) antagonist. The resulting NS1 mutant viruses induced IFN and, as a consequence, were found to be attenuated in vitro and in vivo. The absence of pathogenicity of the NS1 mutants in both BALB/c and C57BL/6 PKR(-/-) mice was confirmed. We also provide evidence that influenza B virus NS1 mutants induce a self-adjuvanted immune response and confer effective protection against challenge with both homologous and heterologous B virus strains in mice.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Vírus da Influenza B
/
Vacinas contra Influenza
/
Proteínas não Estruturais Virais
Limite:
Animals
Idioma:
En
Revista:
J Virol
Ano de publicação:
2008
Tipo de documento:
Article
País de afiliação:
Estados Unidos