Engraftment potential of human placenta-derived mesenchymal stem cells after in utero transplantation in rats.
Hum Reprod
; 24(1): 154-65, 2009 Jan.
Article
em En
| MEDLINE
| ID: mdl-18845668
ABSTRACT
BACKGROUND:
Human placental mesenchymal stem cells (hPMCs) are thought to be multipotent, but their fate after in utero transplantation is not known.METHODS:
hPMCs isolated from term placenta were assessed for their phenotype markers, mutilineage capacity, and immunomodulatory properties. Their engraftment potential was analyzed in a pregnant rat model after in utero transplantation at embryonic day 17. Immunohistochemistry, tracing of labeled cells, fluorescence in situ hybridization and real-time PCR were used to assess post-transplant chimerism.RESULTS:
In vitro, lineage-negative, CD34-negative hPMCs differentiated into osteocytes, adipocytes, hepatocytes and endothelial cells with tube formation, and actively suppressed the rat lymphocyte proliferative response to allogeneic lymphocyte stimulation (P < 0.0001). After in utero transplantation into pregnant rats, a low level of engraftment was achieved in various fetal tissues. Engraftment occurred in more than 60% of the fetal rats. Cells persisted for at least 12 weeks after delivery and evidence was obtained to suggest differentiation into specific lineages, including hepatocytes and hematopoietic cells. However, a greater number of hPMCs migrated to the placenta than to the fetus, thus limiting the degree of cell engraftment in fetal organs.CONCLUSIONS:
We conclude that hPMCs are mutipotent cells that can be engrafted long-term in immunocompetent rats after in utero transplantation.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Placenta
/
Células-Tronco Multipotentes
/
Transplante de Células-Tronco Mesenquimais
/
Embrião de Mamíferos
/
Feto
/
Células-Tronco Mesenquimais
Limite:
Animals
/
Female
/
Humans
/
Pregnancy
Idioma:
En
Revista:
Hum Reprod
Assunto da revista:
MEDICINA REPRODUTIVA
Ano de publicação:
2009
Tipo de documento:
Article
País de afiliação:
Taiwan