Assaying beta-catenin/TCF transcription with beta-catenin/TCF transcription-based reporter constructs.
Methods Mol Biol
; 468: 99-110, 2008.
Article
em En
| MEDLINE
| ID: mdl-19099249
ABSTRACT
Transcription-based reporters have been instrumental in characterizing the Wnt/beta-catenin signaling pathway and will be essential in the search for therapeutics aimed at combating diseases linked to aberrant signaling. In this chapter, we introduce a new improved Wnt/beta-catenin reporter system, beta-catenin-activated reporter (BAR), and its accompanying control reporter system, found unresponsive BAR (fuBAR). Its enhanced sensitivity, increased dynamic range, and lentiviral platform provide a reporter system that will keep pace with the needs of scientists in the field.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Transcrição Gênica
/
Bioensaio
/
Genes Reporter
/
Beta Catenina
/
Fatores de Transcrição TCF
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Methods Mol Biol
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
2008
Tipo de documento:
Article
País de afiliação:
Estados Unidos