Considerations for the processing and analysis of GoldenGate-based two-colour Illumina platforms.
Stat Methods Med Res
; 18(5): 437-52, 2009 Oct.
Article
em En
| MEDLINE
| ID: mdl-19153169
ABSTRACT
Illumina's GoldenGate technology is a two-channel microarray platform that allows for the simultaneous interrogation of about 1,500 locations in the genome. GoldenGate has proved a flexible platform not only in the choice of those 1,500 locations, but also in the choice of the property being measured at them. It retains the desirable properties of Illumina's BeadArrays in that the probes (in this case 'beads') are randomly arranged across the microarray, there are multiple instances of each probe and many samples can be processed simultaneously. As for other Illumina technologies, however, these properties are not exploited as they might be. Here we review the various common adaptations of the GoldenGate platform, review the analysis methods that are associated with each adaptation and then, with the aid of a number of example data sets we illustrate some of the improvements that can be made over the default analysis.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Interpretação Estatística de Dados
/
Análise de Sequência com Séries de Oligonucleotídeos
/
Genômica
Limite:
Humans
Idioma:
En
Revista:
Stat Methods Med Res
Ano de publicação:
2009
Tipo de documento:
Article
País de afiliação:
Reino Unido