Detection and analysis of low-abundance cell-surface biomarkers using enzymatic amplification in microfluidic droplets.
Angew Chem Int Ed Engl
; 48(14): 2518-21, 2009.
Article
em En
| MEDLINE
| ID: mdl-19235824
ABSTRACT
Finding the few Cell-surface proteins are useful disease biomarkers, but current high-throughput methods are limited to detecting cells expressing more than several hundred proteins. Enzymatic amplification in microfluidic droplets (see picture) is a high-throughput method for detection and analysis of cell-surface biomarkers expressed at very low levels on individual human cells. Droplet optical labels allow concurrent analysis of several samples.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Técnicas Analíticas Microfluídicas
/
Proteínas de Membrana
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Revista:
Angew Chem Int Ed Engl
Ano de publicação:
2009
Tipo de documento:
Article
País de afiliação:
Suécia