Dual culture method to determine the relationship of gut bacteria of sandfly (Phlebotomus argentipes) with promastigotes of Leishmania donovani.
J Commun Dis
; 40(2): 133-8, 2008 Jun.
Article
em En
| MEDLINE
| ID: mdl-19301698
ABSTRACT
A simple dual culture agar plating technique has been developed and evaluated for its efficiency in determining the relationship of gut bacteria of sandfly with Leishmania donovani promastigotes. There are about twenty morphologically distinct bacterial colonies have been isolated from the gut homogenate of Phlebotomus argentipes. In dual culture method, each bacterial isolate was inoculated in one half of the plate and the promastigotes of Leishmania was inculcated in the other half by streaking. After incubation, the type of association was determined based on the presence or absence of promastigotes colonies. The reliability of this method was compared with broth dilution method in 96 well plate.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Phlebotomus
/
Bactérias
/
Leishmania donovani
/
Meios de Cultura
/
Trato Gastrointestinal
Tipo de estudo:
Evaluation_studies
Limite:
Animals
/
Humans
Idioma:
En
Revista:
J Commun Dis
Ano de publicação:
2008
Tipo de documento:
Article
País de afiliação:
Índia