Molecular-scale force measurement in a coiled-coil peptide dimer by electron spin resonance.
J Am Chem Soc
; 131(15): 5374-5, 2009 Apr 22.
Article
em En
| MEDLINE
| ID: mdl-19331323
ABSTRACT
A new method for measuring forces between small protein domains based on double electron-electron resonance (DEER) spectroscopy is demonstrated using a model peptide derived from the alpha-helical coiled-coil leucine zipper of yeast transcriptional activator GCN4. The equilibrium distribution of distances between two nitroxide spin labels rigidly attached to the helices of the dimer was determined by DEER and yielded a closing force of 100 +/- 10 pN between monomers, in excellent agreement with theoretical predictions.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Peptídeos
/
Espectroscopia de Ressonância de Spin Eletrônica
/
Fenômenos Químicos
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
J Am Chem Soc
Ano de publicação:
2009
Tipo de documento:
Article
País de afiliação:
Estados Unidos