Antipathogenic properties of green tea polyphenol epigallocatechin gallate at concentrations below the MIC against enterohemorrhagic Escherichia coli O157:H7.
J Food Prot
; 72(2): 325-31, 2009 Feb.
Article
em En
| MEDLINE
| ID: mdl-19350976
ABSTRACT
The inhibitory effects of green tea polyphenol epigallocatechin gallate (EGCG) on virulence phenotypes and gene expression regulated by quorum sensing (QS) in Escherichia coli O157H7 were demonstrated at concentrations of 1 to 100 microg/ml, which are lower than the MIC (539 +/- 22 microg/ml). At 25 microg/ml, the growth rate was not affected, but autoinducer 2 concentration, biofilm formation, and swarm motility decreased to 13.2, 11.8, and 50%, respectively. Survival at 5 days of nematodes (Caenorhabditis elegans) that were fed the pathogen without and with EGCG were 47.1 and 76%, respectively. Real-time PCR data indicated decreased transcriptional level in many quorum sensing-regulated virulence genes at 25 microg/ml. Our results suggest that EGCG at concentrations below itsMIC has significant antipathogenic effects against E. coli O157H7.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Chá
/
Catequina
/
Regulação Bacteriana da Expressão Gênica
/
Caenorhabditis elegans
/
Escherichia coli O157
Limite:
Animals
Idioma:
En
Revista:
J Food Prot
Ano de publicação:
2009
Tipo de documento:
Article