Functional magnetic nanoparticles for biodefense and biological threat monitoring and surveillance.
Anal Chem
; 81(14): 5637-45, 2009 Jul 15.
Article
em En
| MEDLINE
| ID: mdl-19522527
ABSTRACT
A new methodology toward biodefense and threat surveillance by water-dispersible, bactericidal, paramagnetic nanoparticles is presented. The nanoparticles consist of magnetite clusters (approximately 70 nm) modified by polyethyleneimine (PEI) and poly(hexamethylene biguanide) (PHMBG) and are prepared by a two-step procedure at an acceptable cost. The cationic nanoparticles are colloidally stable in water at pH < or = 10, where they sequester DNA or whole microbes. The nanoparticles and bound DNA are captured by high-gradient magnetic separation. Methods of efficient extraction and quantification of the DNA by real-time PCR are developed. Broad bactericidal activity of the nanoparticles is demonstrated at concentrations far below cytotoxicity levels for mammalian cells. The levels of the DNA detection sensitivity obtained in our experiments allow us to project the applicability of the developed method for the detection of DNA molecules of various germs.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Guerra Biológica
/
Nanopartículas
/
Magnetismo
Tipo de estudo:
Screening_studies
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Anal Chem
Ano de publicação:
2009
Tipo de documento:
Article
País de afiliação:
Estados Unidos