Photochemical regulation of restriction endonuclease activity.
Chembiochem
; 10(10): 1612-6, 2009 Jul 06.
Article
em En
| MEDLINE
| ID: mdl-19533711
ABSTRACT
Removal by the light The photochemical regulation of restriction endonucleases, which are important enzymes in molecular biology, has been investigated. Photolabile protecting groups have been installed on DNA substrates and have been demonstrated to inhibit restriction endonuclease activity until removed by UV light irradiation. Interestingly, these groups do not appear to dramatically affect initial binding of the enzyme to the DNA substrate, but rather prevent recognition of the specific cleavage site.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Timidina
/
DNA
/
Enzimas de Restrição do DNA
/
Benzodioxóis
Idioma:
En
Revista:
Chembiochem
Assunto da revista:
BIOQUIMICA
Ano de publicação:
2009
Tipo de documento:
Article
País de afiliação:
Estados Unidos