DNase gamma-dependent and -independent apoptotic DNA fragmentations in Ramos Burkitt's lymphoma cell line.
Biomed Res
; 30(3): 165-70, 2009 Jun.
Article
em En
| MEDLINE
| ID: mdl-19574717
ABSTRACT
DNA fragmentation is a biochemical hallmark of apoptosis. Several endonucleases, including CAD/DFF40 and endonuclease G, are implicated in DNA fragmentation. DNase gamma has also been considered to be one of the enzymes involved, but its role in relation to CAD/DFF40 in apoptosis has not been fully elucidated. Here, we distinguished between DNase gamma-dependent and CAD/DFF40-dependent DNA fragmentations. We found that DNase gamma activities appeared in the late apoptotic phase and accelerated DNA fragmentation. Thus, even if the apoptotic DNA fragmentation is initiated by CAD/DFF40, DNase gamma is required for the more complete digestion of the genomic DNA in dying cells.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Linfoma de Burkitt
/
Apoptose
/
Linhagem Celular Tumoral
/
Endodesoxirribonucleases
/
Fragmentação do DNA
Limite:
Humans
Idioma:
En
Revista:
Biomed Res
Ano de publicação:
2009
Tipo de documento:
Article
País de afiliação:
Japão