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The nucleolar RNA methyltransferase Misu (NSun2) is required for mitotic spindle stability.
Hussain, Shobbir; Benavente, Sandra Blanco; Nascimento, Elisabete; Dragoni, Ilaria; Kurowski, Agata; Gillich, Astrid; Humphreys, Peter; Frye, Michaela.
Afiliação
  • Hussain S; Wellcome Trust Centre for Stem Cell Research, University of Cambridge, England, UK.
J Cell Biol ; 186(1): 27-40, 2009 Jul 13.
Article em En | MEDLINE | ID: mdl-19596847
ABSTRACT
Myc-induced SUN domain-containing protein (Misu or NSun2) is a nucleolar RNA methyltransferase important for c-Myc-induced proliferation in skin, but the mechanisms by which Misu contributes to cell cycle progression are unknown. In this study, we demonstrate that Misu translocates from the nucleoli in interphase to the spindle in mitosis as an RNA-protein complex that includes 18S ribosomal RNA. Functionally, depletion of Misu caused multiple mitotic defects, including formation of unstructured spindles, multipolar spindles, and chromosome missegregation, leading to aneuploidy and cell death. The presence of both RNA and Misu is required for correct spindle assembly, and this process is independent of active translation. Misu might mediate its function at the spindle by recruiting nucleolar and spindle-associated protein (NuSAP), an essential microtubule-stabilizing and bundling protein. We further identify NuSAP as a novel direct target gene of c-Myc. Collectively, our results suggest a novel mechanism by which c-Myc promotes proliferation by stabilizing the mitotic spindle in fast-dividing cells via Misu and NuSAP.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Nucléolo Celular / Metiltransferases / Fuso Acromático Limite: Animals / Humans Idioma: En Revista: J Cell Biol Ano de publicação: 2009 Tipo de documento: Article País de afiliação: Reino Unido

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Nucléolo Celular / Metiltransferases / Fuso Acromático Limite: Animals / Humans Idioma: En Revista: J Cell Biol Ano de publicação: 2009 Tipo de documento: Article País de afiliação: Reino Unido