[Extraction and purification of Atractylodes macrocephala monosaccharide composition].
Zhong Yao Cai
; 32(4): 597-600, 2009 Apr.
Article
em Zh
| MEDLINE
| ID: mdl-19645248
ABSTRACT
OBJECTIVE:
To study the method of Atractylodes macrocephala monosaccharide composition (AMMC)'s extraction, purification and analysis.METHODS:
To extract AMMC with water extraction method and purify it with neutro-alumina and resin adsorption method, to observe removal impurity effect of several methods and determine the technology parameters. To detect monosaccharide's content in AMMC with hydrochloric acid-ultraviolet spectrophotometric determination and Evaporative light scattering detector (ELSD).RESULTS:
After purificated with neutro-alumina, the yield and purity of AMMC were 23% - 25.5% and 56% - 60%, respectively. According to the static decoloring curve of D900, D201 and AB-8 resins, their decolorization effect's rank was D900 > AB-8 > D201. The yield of AMMC purificated with D900 ion exchange resin was above 17%, and the purity of monosaccharide was above 76%.CONCLUSION:
The effect of AMMC purificated with D900 ion exchange resin after water extration is much better, the ideal method for the quantitative analysis of monosaccharide is hydrochloric acid-ultraviolet spectrophotometric determination.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Plantas Medicinais
/
Tecnologia Farmacêutica
/
Atractylodes
/
Resinas de Troca Aniônica
/
Monossacarídeos
Idioma:
Zh
Revista:
Zhong Yao Cai
Assunto da revista:
TERAPIAS COMPLEMENTARES
Ano de publicação:
2009
Tipo de documento:
Article
País de afiliação:
China